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Catalog Number | orb1946636 |
---|---|
Category | Assays and Kits |
Description | Cattle TRH(Thyrotropin Releasing Hormone) ELISA Kit |
Reactivity | Bovine |
Range | 7.82-500 pg/mL |
Target | TRH |
Sensitivity | 2.63 pg/mL |
Alternative names | TRF; Thyrotropin-Releasing Factor; Thyroliberin; P Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(Cattle TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(Cattle TRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone(Cattle TRH) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Bovine | |
7.82-500 pg/mL | |
2.63 pg/mL |
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