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14-3-3 η rabbit pAb

Catalog Number: orb770398

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb770398
CategoryAntibodies
DescriptionRabbit polyclonal antibody to 14-3-3 Eta
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human 14-3-3 eta. AA range:51-100
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
ConjugationUnconjugated
MW42
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-YWHAH antibody, anti-YWHA1 antibody, anti-14-
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NoteFor research use only
Expiration Date12 months from date of receipt.
14-3-3 η rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

14-3-3 η rabbit pAb

Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is blocked with the synthesized peptide.

14-3-3 η rabbit pAb

Western blot analysis of lysates from Jurkat cells, using 14-3-3 eta Antibody. The lane on the right is blocked with the synthesized peptide.