14-3-3 ζ rabbit pAb

• Catalog Number: orb764418
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 14-3-3 zeta
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 28
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-YWHAZ antibody, anti-14-3-3 protein zeta/delt
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of COLO205 cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of 293T HELA using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.