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| Catalog Number | orb764378 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to 14-3-3 zeta (phospho-Ser58) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 30 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-YWHAZ antibody, anti-14-3-3 protein zeta/delt Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 zeta (Phospho-Ser58) Antibody.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
