ZO-1/TJP1 Antibody

• Catalog Number: orb1294380
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZO-1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human ZO-1
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Conjugation: Unconjugated
• Target: ZO-1 / TJP1
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-ZO-1 antibody - at 1/500 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294380 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ZO-1 with orb1294380 at 1/100.

All lanes: Anti-ZO-1 antibody - at 1/500 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294380 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294380 Anti-ZO-1 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294380 Anti-ZO-1 antibody (Green) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.