Zebrafish 5-HT(5-Hydroxytryptamine) ELISA Kit

• Catalog Number: orb1946645
• Category: Assays and Kits
• Description: Zebrafish 5-HT(5-Hydroxytryptamine) ELISA Kit
• Reactivity: Zebrafish
• Range: 14.07-900 ng/mL
• Target: 5-HT
• Sensitivity: 3.97 ng/mL
• Alternative names: 5HT; Serotonin; N-Methyl-Gamma
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine(5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates
• Expiration Date: Please enquire.