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Catalog Number | orb1238535 |
---|---|
Category | Antibodies |
Description | ZEB2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2.The immunogen is located within the last 50 amino acids of ZEB2. |
Concentration | 1 mg/mL |
Dilution range | ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 134 kDa Observed: 135 kDa |
Target | ZEB2 |
UniProt ID | O60315 |
NCBI | AAI27103 |
Storage | ZEB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | ZEB2 Antibody: SIP1, SIP-1, ZFHX1B, HSPC082, SMADI Read more... |
Note | For research use only |
Application notes | ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/ml. Red: ZEB2 Antibody (orb1238535) Blue: DAPI staining.
Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.
IHC, WB | |
Bovine, Equine, Guinea pig, Mouse, Porcine, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, IHC-Fr, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Primate, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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