ZEB1/NIL2A Rabbit Polyclonal Antibody

• Catalog Number: orb6954
• Category: Antibodies
• Description: ZEB1/NIL2A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ZEB1 (211-320/1124aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 124 kDa
• Target: ZEB1
• UniProt ID: P37275
• RRID: AB_10936750
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AREB 6; AREB6; BZP; Delta crystallin enhancer bind
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: H9C2 cells, Isotype Control Antibody: Rabbit IgG-PE, Primary Antibody, dilution: 5 µl in 100 µl 1X PBS containing 0.5% BSA.

Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-ZEB1/NIL2A antibody (orb6954), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZEB1) Polyclonal Antibody, Unconjugated (orb6954) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZEB1) Polyclonal Antibody, Unconjugated (orb6954) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: (mo) H9C2 (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody catalog number: orb6954, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-ZEB1/NIL2A (orb6954) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-ZEB1/NIL2A (orb6954) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-ZEB1/NIL2A (orb6954) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.