ZC3H12A Antibody

• Catalog Number: orb1672348
• Category: Antibodies
• Description: ZC3H12A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Predicted Reactivity: Gallus, Monkey, Porcine, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-ZC3H12A antibody (orb1672348) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A. The immunogen is located within the last 50 amino acids of ZC3H12A.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 66 kD Observed: 66 kD
• Target: ZC3H12A
• UniProt ID: Q5D1E8
• NCBI: Q5D1E7.2
• Storage: ZC3H12A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation in Human Lung. Loading: 15 µg of lysate per lane. Antibodies: ZC3H12A orb1672348, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 2 µg/mL. Lane 2: 4 µg/mL.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with orb1672348 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZC3H12A in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.