ZAP70 (phospho-Y315) antibody

• Catalog Number: orb214753
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZAP70
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y315 of human ZAP70 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ZAP70
• Entrez: 7535, 22637
• UniProt ID: P43403, P43404
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SRK antibody, anti Tyrosine-protein kinase ZA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZAP70 (Phospho-Y315) expression in Jurkat H2O2-treated (A), HeLa H2O2-treated (B), Ramos lambda phosphatase-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)

Immunohistochemical analysis of ZAP70 (Phospho-Y315) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.