ZAP70 Antibody

• Catalog Number: orb749850
• Category: Antibodies
• Description: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2F3.2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ZAP-70 antibody is available for research use only.
• UniProt ID: P43403
• Storage: Store the ZAP-70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZAP-70 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP-70 antibody (green, clone 2F3.2) and Reddot nuclear stain (red).

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with ZAP-70 antibody (clone 2F3.2).

Western blot testing of human Jurkat cell lysate with ZAP-70 antibody (clone 2F3.2).

Flow testing of PFA-fixed Jurkat cells with ZAP-70 antibody (clone 2F3.2); Red=isotype control, Blue=ZAP70 antibody.

SDS-PAGE analysis of purified, BSA-free ZAP-70 antibody (clone 2F3.2) as confirmation of integrity and purity.