ZAP70 Antibody

• Catalog Number: orb699581
• Category: Antibodies
• Description: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rZAP70/2046
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 247-382 from the human protein was used as the immunogen for the recombinant ZAP70 antibody.
• Dilution range: ELISA: 2-4ug/ml for coating (order Ab without BSA),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant ZAP70 antibody is available for research use only.
• UniProt ID: P43403
• Storage: Store the recombinant ZAP70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant ZAP70 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with recombinant ZAP70 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymphoma with recombinant ZAP70 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant ZAP70 antibody as confirmation of integrity and purity.