ZAP70 antibody

• Catalog Number: orb1473894
• Category: Antibodies
• Description: Mouse monoclonal antibody to ZAP70
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of human ZAP70. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ZAP70
• Entrez: 7535, 22637
• UniProt ID: P43403, P43404
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZAP70 expression in Jurkat (A), MOLT4 (B), mouse thymus (C) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)

Immunofluorescent analysis of ZAP70 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Jurkat cells using Anti-ZAP70 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.