XRCC5 Antibody

• Catalog Number: orb1257285
• Category: Antibodies
• Description: XRCC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human XRCC5 (NP_066964.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 86kDa
• Target: XRCC5
• UniProt ID: P13010
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: XRCC5, KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using XRCC5 antibody (orb1257285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human breast cancer using XRCC5 Antibody (orb1257285) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC5 Antibody (orb1257285) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using XRCC5 antibody (orb1257285). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using XRCC5 antibody (orb1257285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.