XRCC3 Antibody

• Catalog Number: orb1258267
• Category: Antibodies
• Description: XRCC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37kDa
• Target: XRCC3
• UniProt ID: O43542
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: DNA repair protein XRCC3, X-ray repair cross-compl
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (orb1258267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (orb1258267) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (orb1258267) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (orb1258267) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using XRCC3 antibody (orb1258267). Blue: DAPI for nuclear staining.