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Catalog Number | orb750181 |
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Category | Antibodies |
Description | Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | WT1/1434R |
Tested applications | IHC-P |
Reactivity | Human, Rat |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant protein was used as the immunogen for the recombinant WT1 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant WT1 antibody is available for research use only. |
UniProt ID | P19544 |
Storage | Store the recombinant WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human mesothelioma tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE rat testis tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
SDS-PAGE analysis of purified, BSA-free recombinant WT1 antibody (clone WT1/1434R) as confirmation of integrity and purity.
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