Western Blot Kit

• Catalog Number: orb342271
• Category: Assays and Kits
• Description: Western Blot Kit
• Tested applications: WB
• Reactivity: Mouse
• Concentration: n/a
• Dilution range: WB: 1:20,000
• Form/Appearance: n/a
• Purity: This kit contains: anti-mouse peroxidase, mouse IgG control, BSA, tween-20, and Femtomax, along with the protocol and additional supplies. See kit insert for complete details and instructions.
• Conjugation: Unconjugated
• Storage: See kit insert for complete instructions.
• Buffer/Preservatives: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
• Alternative names: Western Blotting Kit, Chemiluminescent Kit, Peroxi
• Note: For research use only
• Application notes: Biorbyt’ Chemiluminescent Western Blot Kit, namely our FemtoMax Chemiluminescent Substrate Kit for Western Blotting, combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol substrate. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 and is stable for at least 1 year when stored at +4°C.
• Expiration Date: Please enquire.

Chemiluminescent Western Blot Kit shows super sensitive signal. Known amounts of GST were spiked into a HeLa lysate followed by separation by SDS-PAGE using a 4-20 % gradient gel. Proteins were transferred to a Protran B85 membrane for 1 hour at 100 mV. The membrane was blocked with 5% BLOTTO (p/n orb348624) in TTBS for 1 h at 4 °C. The blot was probed for 40 min with 1:1000 dilution of Mab anti-GST (p/n orb344400) followed by HRP anti-Mouse IgG (p/n orb347385) at 1:10000 dilution in blocking buffer for 1 h at 4°C.