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Catalog Number | orb527041 |
---|---|
Category | Antibodies |
Description | WEE1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human WEE1 (ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 72 kDa |
UniProt ID | P30291 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Wee1-like protein kinase; WEE1hu; Wee1A kinase; WE Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x10^6 cells |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SiHa cells using anti-WEE1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of WEE1 using anti-WEE1 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human A431 cell;4:human U2OS cell.
IF analysis of WEE1 using anti-WEE1 antibody. WEE1 was detected in immunocytochemical section of U20S cells.
IHC analysis of WEE1 using anti-WEE1 antibody. WEE1 was detected in paraffin-embedded section of human mammary cancer tissue.
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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