VWF Antibody

• Catalog Number: orb1274260
• Category: Antibodies
• Description: VWF Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: VWF/1859R
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG, k
• Immunogen: Amino acids 1815-1939 from the human protein were used as the immunogen for this recombinant vWF antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlImmunoprecipitation: 1-2ug/500ug lysateImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RTThe optimal dilution of the recombinant vWF antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VWF
• UniProt ID: P04275
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlImmunoprecipitation: 1-2ug/500ug lysateImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RTThe optimal dilution of the recombinant vWF antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with recombinant vWF antibody (clone VWF/1859R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of 1) partial recombinant protein and 2) human lung lysate with recombinant vWF antibody. Predicted molecular weight ~250 kDa.