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Catalog Number | orb1260356 |
---|---|
Category | Antibodies |
Description | VTN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 399-478 of human VTN (NP_000629.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 70kDa |
Target | VTN |
UniProt ID | P04004 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Vitronectin, VN, S-protein, Serum-spreading factor Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of human serum, using VTN antibody (orb1260356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using VTN antibody (orb1260356) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using VTN antibody (orb1260356) at dilution of 1:100 (40x lens).
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