VTCN1 Antibody / B7H4

• Catalog Number: orb640004
• Category: Antibodies
• Description: T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B7H4/1788
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant human partial protein was used as the immunogen for this B7-H4 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This B7-H4 antibody is available for research use only.
• UniProt ID: Q7Z7D3
• Storage: Store the B7-H4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788, green) and Reddot nuclear stain (red).

Flow cytometry testing of fixed human SK-BR-3 cells with B7-H4 antibody (clone B7H4/1788); Red=isotype control, Blue= B7-H4 antibody.

IHC staining of FFPE human testicular carcinoma with B7-H4 antibody (clone B7H4/1788). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HCT-116 cell lysate with B7-H4 antibody. Predicted molecular weight: ~31 kDa.

SDS-PAGE analysis of purified, BSA-free B7-H4 antibody (clone B7H4/1788) as confirmation of integrity and purity.