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Catalog Number | orb1246407 |
---|---|
Category | Antibodies |
Description | VPS16 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-KESQKADEYLREIQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7kDa according to NP_072097.2). Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 100kDa |
Target | VPS16 |
UniProt ID | Q9H269 |
NCBI | NP_072097.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | VPS16, vacuolar protein sorting 16 homolog (S. cer Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7kDa according to NP_072097.2). Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246407 (0.1 ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246407 (0.1 ug/ml) staining of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Porcine, Rat | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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