VISTA Antibody / GI24

• Catalog Number: orb1151753
• Category: Antibodies
• Description: Mouse monoclonal antibody to VISTA
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VISTA/2864
• Tested applications: ELISA
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human VISTA protein was used as the immunogen for the VISTA antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating)
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This VISTA antibody is available for research use only.
• UniProt ID: Q9H7M9
• Storage: Aliquot the VISTA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the VISTA antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using VISTA antibody (clone VISTA/2864). These results demonstrate the foremost specificity of the VISTA/2864 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free VISTA antibody (clone VISTA/2864) as confirmation of integrity and purity.