Vinculin rabbit pAb

• Catalog Number: orb766575
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vinculin
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 123
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-VCL antibody, anti-Vinculin antibody, anti-Me
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.