Vinculin Antibody

• Catalog Number: orb606673
• Category: Antibodies
• Description: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and alpha-Actinin. In addition, vinculin, Talin and alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VCL/2575
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 174-322 from the human protein was used as the immunogen for this Vinculin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Vinculin antibody is available for research use only.
• UniProt ID: P18206
• Storage: Store the Vinculin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Vinculin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of 1) human A431, 2) human K562 and 3) mouse NIH 3T3 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.

Western blot testing of human 1) U-87 MG and 2) ThP-1 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.

IHC testing of FFPE human testis tissue with Vinculin antibody (clone VCL/2575). HIER: boil tissue sections in pH9 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Vinculin antibody (clone VCL/2575). These results demonstrate the foremost specificity of the VCL/2575 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Vinculin antibody (clone VCL/2575) as confirmation of integrity and purity.