Vinculin Antibody

• Catalog Number: orb1826255
• Category: Antibodies
• Description: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and Alpha-Actinin. In addition, vinculin, Talin and Alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: VCL/8533R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein (within amino acids 174-322) from the human protein was used as the immunogen for the Vinculin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This Vinculin antibody is available for research use only.
• UniProt ID: P18206
• Storage: Aliquot the Vinculin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with Vinculin antibody (clone VCL/8533R) at 2 ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Vinculin antibody (clone VCL/8533R) as confirmation of integrity and purity.