Vimentin Antibody

• Catalog Number: orb420717
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vimentin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB41034
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Recombinant Protein
• Dilution range: WB: 1:2000, IHC-P: 1:50, FACS: 1:50, IF/ICC: 1:50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 53652
• Target: VIM
• UniProt ID: P08670
• NCBI: NP_003371.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Vimentin, VIM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-VIME Antibody at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: NIH/3T3 whole cell lysate. Lane 7: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-VIME Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: NIH/3T3 whole cell lysate. Lane 7: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.