Vimentin Antibody

• Catalog Number: orb2636747
• Category: Antibodies
• Description: Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: VIM/6430R
• Tested applications: IHC-P
• Reactivity: Bovine, Canine, Equine, Feline, Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Vimentin antibody is available for research use only.
• UniProt ID: P08670
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse small intestine tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE horse lung tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cow brain tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human U-87 MG cell lysate with recombinant Vimentin antibody. Predicted molecular weight ~53 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6430R) as confirmation of integrity and purity.