Vimentin antibody

• Catalog Number: orb1151550
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rVIM/6431
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant VIM antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant VIM antibody is available for research use only.
• UniProt ID: P08670
• Storage: Aliquot the recombinant VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant VIM antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with recombinant VIM antibody (clone rVIM/6431). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6431). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6431). Predicted molecular weight ~53 kDa.