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Catalog Number | orb1151549 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Vimentin |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rVIM/6575 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant full-length human Vimentin protein was used as the immunogen for the Vimentin antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Vimentin antibody is available for research use only. |
UniProt ID | P08670 |
Storage | Aliquot the Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Vimentin antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of human U-87 MG cell lysate using Vimentin antibody (clone rVIM/6575). Predicted molecular weight ~53 kDa.
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Vimentin antibody (clone rVIM/6575). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone rVIM/6575) as confirmation of integrity and purity.
IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IP, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Equine, Feline, Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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