Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody

• Catalog Number: orb388774
• Category: Antibodies
• Description: Mouse monoclonal antibody to VIL1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1325
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary))
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 1-2 μg/ml, IHC-P: 0.25-0.5 μg/ml
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 93kDa
• Target: VIL1
• Entrez: 7429
• UniProt ID: P09327
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: VIL1; Villin-1; Villin1
• Note: For research use only
• Application notes: Villin (GI-Mucosal & Urogenital Brush Border Marker)
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Rectum tissue using VIL1 antibody

Western blot analysis of A549, HepG2 & HCT116 Cell Lysates using VIL1 antibody

Immunohistochemical staining of human Colon tissue using VIL1 antibody