Villin Antibody

• Catalog Number: orb2640156
• Category: Antibodies
• Description: Recognizes a protein of ~93kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1314
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Villin antibody is available for research use only.
• UniProt ID: P09327
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the Villin antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1314). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon with Villin antibody (clone VIL1/1314). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) A549, 2) HepG2 and 3) HCT-116 cell lysate with Villin antibody (clone VIL1/1314). Expected molecular weight ~93 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1314). Confirmation of Integrity and Purity of the Antibody.