VhH

• Catalog Number: orb750529
• Category: Antibodies
• Description: VhH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Camelus
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.
• Concentration: 80mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-VhH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-Vhh antibody detects recombinant VhH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-VhH Antibody, single-domain antibody (
• Note: For research use only
• Application notes: Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 µg lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.

Western Blot Results of Rabbit Anti-VhH Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: Llama IgG1 isotype control (p/n orb343832) reduced [0.05 µg]. Lane 3: Llama IgG2 isotype control (p/n orb343834) reduced [0.05 µg]. Lane 4: Llama IgG3 isotype control (p/n orb343833) reduced [0.05 µg]. Lane 5: VhH Control Protein reduced [0.05 µg]. Primary Antibody: Anti-VhH [Rabbit] Antibody at 1:1000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG [Goat] Peroxidase Conjugated (p/n orb347673) at 1:40000 for 30 mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1 hr at RT. Expected: recognizes the VhH protein ~15 kDa and detects the llama IgG1, IgG2 and IgG3 proteins. Exposure: 6.8 sec.