VhH

• Catalog Number: orb750528
• Category: Antibodies
• Description: VhH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Camelus
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.
• Concentration: 80mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-VhH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-Vhh antibody detects recombinant VhH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-VhH Antibody, single-domain antibody (
• Note: For research use only
• Application notes: Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 µg lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.

Western Blot Results of Rabbit Anti-VhH Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: Llama IgG1 isotype control (p/n orb343832) reduced [0.05 µg]. Lane 3: Llama IgG2 isotype control (p/n orb343834) reduced [0.05 µg]. Lane 4: Llama IgG3 isotype control (p/n orb343833) reduced [0.05 µg]. Lane 5: VhH Control Protein reduced [0.05 µg]. Primary Antibody: Anti-VhH [Rabbit] Antibody at 1:1000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG [Goat] Peroxidase Conjugated (p/n orb347673) at 1:40000 for 30 mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1 hr at RT. Expected: recognizes the VhH protein ~15 kDa and detects the llama IgG1, IgG2 and IgG3 proteins. Exposure: 6.8 sec.