VhH antibody

• Catalog Number: orb420273
• Category: Antibodies
• Description: VhH antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Camelus
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.
• Concentration: 1.3 mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-VhH is directed against the llama VhH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-Vhh antibody detects recombinant VhH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-VhH Antibody, single-domain antibody (
• Note: For research use only
• Application notes: Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-VhH Antibody detecting VhH. Lane 1: MW Ladder Opal pre-stained. Lane 2: VhH. Load: 0.50 µg per lane. Primary Antibody: Anti-VhH Antibody (p/n orb420272) at 1 ug/ml for overnight at 4˚C. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: orb348637 at RT for 30 min. Predicted/Observed size: ~15 kDa.

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 µg lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.

Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein. Lane 3: Llama IgG1 protein (p/n orb343832). Lane 4: Llama IgG2 protein (p/n orb343834). Lane 5: Llama IgG3 protein (p/n orb343833). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (orb347654) rabbit secondary antibody at 1:70000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: Llama IgG1 protein (p/n orb343832). Lane 3: Llama IgG2 protein (p/n orb343834). Lane 4: Llama IgG3 protein (p/n orb343833). Lane 5: VhH protein. Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:40000 for 45 min at RT. Block: BlockOut (p/n orb348637) overnight at 4°C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.