VhH antibody

• Catalog Number: orb420272
• Category: Antibodies
• Description: VhH antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Camelus
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VhH camelid domain protein.
• Concentration: 1.08 mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-VhH is directed against the llama VhH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-Vhh antibody detects recombinant VhH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-VhH Antibody, single-domain antibody (
• Note: For research use only
• Application notes: Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-VhH Antibody detecting VhH. Lane 1: MW Ladder Opal pre-stained. Lane 2: VhH. Load: 0.50 µg per lane. Primary Antibody: Anti-VhH Antibody (p/n orb420272) at 1 ug/ml for overnight at 4˚C. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: orb348637 at RT for 30 min. Predicted/Observed size: ~15 kDa.

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 µg lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.

Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein. Lane 3: Llama IgG1 protein (p/n orb343832). Lane 4: Llama IgG2 protein (p/n orb343834). Lane 5: Llama IgG3 protein (p/n orb343833). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (orb347654) rabbit secondary antibody at 1:70000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: Llama IgG1 protein (p/n orb343832). Lane 3: Llama IgG2 protein (p/n orb343834). Lane 4: Llama IgG3 protein (p/n orb343833). Lane 5: VhH protein. Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:40000 for 45 min at RT. Block: BlockOut (p/n orb348637) overnight at 4°C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.