VGF Antibody

• Catalog Number: orb1238617
• Category: Antibodies
• Description: VGF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF.The immunogen is located within the last 50 amino acids of VGF.
• Concentration: 1 mg/mL
• Dilution range: VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 68 kDa Observed: 70 kDa
• Target: VGF
• UniProt ID: O15240
• NCBI: NP_003369
• Storage: VGF antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: VGF Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: VGF Antibody: Neurosecretory protein VGF, NERP-1
• Note: For research use only
• Application notes: VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 µg/ml.

Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 µg/mL.