You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1272271 |
---|---|
Category | Antibodies |
Description | Vegfa Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF (murine VEGF). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0 2.0 μg/mL of this antibody is required. ELISA:To detect mVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mVEGF.Sandwich:To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine VEGFas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Western Blot:To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Vegfa |
UniProt ID | Q00731 |
NCBI | Q00731 |
Storage | VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Vpf, Vegf, Vascular endothelial growth factor A, V Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine VEGF (orb1272270) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Gallus, Guinea pig, Human, Mouse, Porcine, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
Blocking, ELISA, FC, IHC, IP, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating