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Catalog Number | orb1272688 |
---|---|
Category | Antibodies |
Description | Vegfa Antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Rat |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Rat VEGF specific antibody was purified by affinity chromatography and then biotinylated. |
Concentration | batch dependent |
Dilution range | ELISA:Sandwich:To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat VEGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF. Western Blot:To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | Vegfa |
UniProt ID | P16612 |
NCBI | P16612 |
Storage | VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | VPF, Vegf, VEGF-A, VEGF164, Vascular endothelial g Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat VEGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF. Western Blot:To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Rat VEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat VEGF (orb1272689) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat VEGF.
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