VEGF121 Recombinant Protein

Catalog Number: orb1230927

Catalog Number	orb1230927
Category	Proteins
Description	VEGF121 Recombinant Protein
Form/Appearance	Lyophilized
Purity	Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Target	VEGFA
UniProt ID	P15692
Protein Sequence	APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
Source	E. coli
Biological Activity	Biological activity was determined by the dose - dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2 - 0.4 ng/mL.
NCBI	NP_001028928.1
Storage	The lyophilized VEGF121 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted VEGF121 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing VEGF121 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Alternative names	VPF, VEGF, MVCD1, Vascular endothelial growth fact Read more...
Note	For research use only
Expiration Date	6 months from date of receipt.

Applications:	WB
Purity:	> 95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121.
MW:	14 kDa (monomer)
Source:	HEK293 cells

Applications:	ELISA, WB
Purity:	> 95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
MW:	15 kDa
Source:	HEK293 cells

Purity:	Greater than 95% as determined by reducing SDS-PAGE. Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
MW:	14.2 kD
Source:	E. coli

MW:	28.2 kDa, observed by non-reducing SDS-PAGE.
Source:	Escherichia coli.