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VEGF121 Recombinant Protein
Catalog Number: orb1230504
| Catalog Number | orb1230504 |
|---|---|
| Category | Proteins |
| Description | VEGF121 Recombinant Protein |
| Form/Appearance | Lyophilized |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses. |
| Target | VEGFA |
| UniProt ID | P15692 |
| Protein Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R |
| Source | E. coli |
| Biological Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/mL. |
| NCBI | NP_001028928.1 |
| Storage | The lyophilized VEGF121 recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted VEGF121 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing VEGF121 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
| Alternative names | VPF, VEGF, MVCD1, Vascular endothelial growth fact Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Biological activity was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/mL. Blue = VEGF165 Rec Protein (Cat No: orb1230928), Pink = WHO Human VEGF Rec Protein, Green = VEGF121 Rec Protein (Animal Free) (Cat No: orb1230504)
- Item 1 of 1
Human VEGF121 Protein, hFc Tag [orb2321385]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg - Item 1 of 1
VEGF 121 Recombinant Protein [orb1227837]
WB
> 95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121.
14 kDa (monomer)
HEK293 cells
0.02 mg - Item 1 of 1
VEGF121 Recombinant Protein [orb1227715]
ELISA, WB
> 95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
15 kDa
HEK293 cells
0.05 mg 
VEGF121 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960084]
98.00%
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgVEGF121 Protein, Human, Recombinant (His & Avi) [orb1960085]
98.00%
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
