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Catalog Number | orb1227715 |
---|---|
Category | Proteins |
Description | VEGF121 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Dilution range | This recombinant protein can be used for E, WB. For research use only. |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
MW | 15 kDa |
Target | VEGF121 |
UniProt ID | P15692 |
Protein Sequence | Ala 27 - Arg 147 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized VEGFR1/R2-Fc at 1 ug/mL can bind Human VEGF121, His Tag with a linear range of 0.2-6.2 ng/mL. |
NCBI | P15692 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for E, WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Immobilized VEGFR1/R2-Fc at 1 ug/ml can bind Human VEGF121, His Tag with a linear range of 0.2-6.2 ng/mL.
WB | |
> 95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121. | |
14 kDa (monomer) | |
HEK293 cells |
Greater than 98% by SDS-PAGE gel and HPLC analyses. | |
E. coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
14.2 kD | |
E. coli |
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). | |
E. coli |
28.2 kDa, observed by non-reducing SDS-PAGE. | |
Escherichia coli. |
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