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Catalog Number | orb1227837 |
---|---|
Category | Proteins |
Description | VEGF 121 Recombinant Protein |
Tested applications | WB |
Tag | Tag free |
Dilution range | This recombinant protein can be used for WB. For research use only. |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121. |
MW | 14 kDa (monomer) |
Target | VEGFA |
UniProt ID | P15692 |
Protein Sequence | Ala 27 - Arg 147 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized ActiveMax Human VEGF121 at 2 ug/mL can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 ug/mL. |
NCBI | NP_001020541 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | VEGF121, VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEG Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human VEGF121, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Greater than 95% as determined by reducing SDS-PAGE. | |
14.2 kD | |
E. coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
15.1 kD | |
Human Cells |
> 97% by SDS-PAGE. | |
KMP1027, Recombinant Human VEGF 121 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala207-Arg327 (Lys321Asn)) of human VEGF 121 (Accession #NP_001020541.2). |
> 95% by SDS-PAGE. | |
KMP1359, Recombinant human VEGF 121 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala27-Arg147) of human VEGF 121 (Accession #NP_001165099.1) fused with a 6×His Tag at the N-terminus. |
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