VC(Vitamin C) ELISA Kit

• Catalog Number: orb1946702
• Category: Assays and Kits
• Description: VC(Vitamin C) ELISA Kit
• Range: 625-40000 ng/mL
• Target: VC
• Sensitivity: 192.1 ng/mL
• Alternative names: AA; L-Ascorbic Acid; Ascorbate
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin C(VC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin C(VC). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin C(VC) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma and other biological fluids
• Expiration Date: Please enquire.