VCAM-1 antibody

• Catalog Number: orb1294364
• Category: Antibodies
• Description: Rabbit polyclonal antibody to VCAM-1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human VCAM-1 / CD106
• Dilution range: Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200
• Conjugation: Unconjugated
• Target: VCAM-1 / CD106 (Extracellular)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294364 Anti-VCAM-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.

Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.

VCAM-1/CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling VCAM-1/CD106 with orb1294364 at 1/100.