Vazyme - T7 Endonuclease I

• Catalog Number: EN303-01
• Category: Tools
• Description: T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5' to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.
• Storage: Store at -20°C for up to 24 months.
• Note: For research use only
• Application notes: High detection sensitivity; Products can be directly detected by electrophoresis
• Expiration Date: 12 months from date of receipt.