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| Catalog Number | PD101-01 |
|---|---|
| Category | Tools |
| Description | 2 × Taq Plus Master Mix (Dye Plus) in this kit contains Taq Plus DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification, thereby reducing pipetting operations, significantly controlling cross-contamination among samples, and improving detection throughput and reproducibility of results. The amplification system contains protective agents that keep 2 × Taq Plus Master Mix (Dye Plus) stable in activity after repeated freezing and thawing. 2 × Taq Plus Master Mix (Dye Plus) contains loading buffer, PCR products can be directly loaded for electrophoresis after the reaction. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is also compatible with ClonExpress and TOPO cloning kits (Biorbyt #C112/C113/C115/C601). |
| Storage | 1 × Mouse tissue Lysis Buffer, store at 2 ~ 8°C; Other components, store at -30 ~ -15°C; Transport at ≤0°C. |
| Background | Only available for the UK and Republic of Ireland |
| Note | For research use only |
| Application notes | Mouse-Direct PCR: No need for DNA purification steps; crude Samples can be added directly to PCR Convenient operation: Master Mix format with premixed gel loading dye; reduces sample handling and allows direct loading on gel Special optimization: Lysis solution based on proteinase K; suitable for rapid genotyping of mouse Broad compatibility: Suitable for ≤ 2kb fragments; up to 4-plex amplification |
| Expiration Date | 12 months from date of receipt. |
