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Catalog Number | orb1246807 |
---|---|
Category | Antibodies |
Description | USP20 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-HGEQKIEAETRAV |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Tonsil lysates (calculated MW of 102kDa according to NP_006667.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | USP20 |
UniProt ID | Q9Y2K6 |
NCBI | NP_006667.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | USP20, VDU2, ubiquitin specific protease 20, KIAA1 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Tonsil lysates (calculated MW of 102kDa according to NP_006667.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246807 (3 ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
orb1246807 (1 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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