Uroplakin 3B Antibody / UPK3B

• Catalog Number: orb1824727
• Category: Antibodies
• Description: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UPK3B/3270
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the UPK3B antibody.
• Dilution range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This UPK3B antibody is available for research use only.
• UniProt ID: Q9BT76
• Storage: Aliquot the UPK3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human bladder tissue with UPK3B antibody (clone UPK3B/3270). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder tissue with UPK3B antibody (clone UPK3B/3270). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using UPK3B antibody (clone UPK3B/3270) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free UPK3B antibody (clone UPK3B/3270) as confirmation of integrity and purity.