Urease antibody (Peroxidase)

• Catalog Number: orb344911
• Category: Antibodies
• Description: Urease antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IP, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Urease [Jack Bean]
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:500 - 1:2,000, IP: User Optimized, WB: 1:500
• Form/Appearance: Lyophilized
• Purity: Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P07374
• NCBI: AAA83831.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Urease Antibody HRP Conjugation, Perox
• Note: For research use only
• Application notes: Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Rabbit Anti-Urease Antibody Peroxidase Conjugated. Urease (1) 33.33 ng, (2) 11.11 ng, (3) 3.70 ng, (4) 1.23 ng. Primary Antibody: Anti-Urease HRP at 1.0 µg/mL for 1 hr at RT. Secondary Antibody: none. Blocking: BlockOut Buffer (orb348644) for 30 mins RT. Exposure: 5 secs.